MHC Gene Polymorphism in Bangladeshi Patients with Vitiligo- a laboratory based cross-sectional observational study

Abstract

Background: Vitiligo is a common depigmenting and disfiguring skin disease. Association of Human Leukocyte Antigen (HLA) and vitiligo is well known and several HLA studies across the population worldwide are done and found to be important in prediction of disease susceptibility.

Aims: To investigate whether any HLA alleles were associated with Vitiligo among Bangladeshi patients.

Methods: It was a cross sectional study, conducted in Bangabandhu Sheikh Mujib Medical University (BSMMU) among 32 clinically diagnosed patients of vitiligo and 30 healthy controls. With all aseptic precaution 2 ml of venous blood was collected in ethylene diaminetetraceticacid (EDTA) containing tube and stored at -20 c until testing. DNA extraction was carried out according to manufacturer’s instructions and were investigated for their HLA genotype. In this study HLA class I and class II typing was done by amplification of extracted DNA by PCR based technique with Micro Sequence-Specific Primers (SSP). Then amplified DNA was run in 2% agarose gel and visualized by UV light. Presence of band of specific PCR product was interpreted with the worksheet supplied by manufacturer.

Results: : Significantlyhigher frequency of HLA A*33(01) and HLA B*44(02) of MHC class-I alleles and DRB1*07(01) and DQB1*02(01) of class II alleles were reported from vitiligo patients.

Limitatioons: Small sample size.

Conclusions:  Increased frequencies of some alleles of HLA class I and class ll antigens in patients showed a notable association of MHC genes with manifestation of vitiligo from Bangladeshi patients.

Keywords: vitiligo, HLA, Polymorphism